Table 3 Results for PERMANOVAs applied to Bray–Curtis transformed microbiome data based on a 16S rRNA amplicons and b shotgun metagenomics.

From: Composition and metabolic potential of microbiomes associated with mesopelagic animals from Monterey Canyon

Source of variation

df

SS

R2

p

pDispersion

a. 16S rRNA amplicons

 Host

10

7.4976

0.49404

0.001

0.052

 Residuals

26

7.6784

0.50596

  

 Diet

2

1.3922

0.09174

0.007

0.243

 Residuals

34

13.7838

0.90826

  

 Migration

2

1.8344

0.12088

0.002

0.253

 Residuals

34

13.3416

0.87912

  

 Depth

4

3.2905

0.21683

0.001

0.001

 Residuals

32

11.8855

0.78317

  

b. Metagenomics

 Host

6

3.2255

0.90916

0.001

0.002

 Residuals

11

0.3223

0.09084

  

 Diet

2

1.3185

0.37164

0.001

0.101

 Residuals

15

2.2292

0.62836

  

 Migration

1

0.5324

0.15008

0.007

0.869

 Residuals

16

3.0153

0.84992

  

 Depth

3

1.6944

0.47760

0.001

0.001

 Residuals

14

1.8533

0.52240

  
  1. P values for permutational MANOVA and permutation tests for homogeneity of multivariate dispersions are shown. Significant tests (p ≤ 0.05) are indicated in bold.
  2. df degrees of freedom, SS sum of squares, R2coefficient of determination (proportional explained variation), p p value.