Table 4 PERMANOVAs assessing abundance of metabolic categories in relation to host species, depth, diet and diel vertical migration based on a 16S rRNA amplicon data and b shotgun metagenomic data.

From: Composition and metabolic potential of microbiomes associated with mesopelagic animals from Monterey Canyon

Source of variation

df

SS

R2

p

pDispersion

16S rRNA data

 Host

10

2.5220

0.4591

0.001

0.157

 Residuals

26

2.9712

0.5409

  

 Diet

2

0.5843

0.1064

0.027

0.114

 Residuals

34

4.9089

0.8936

  

 Migration

2

0.5892

0.1073

0.025

0.300

 Residuals

34

4.9041

0.8928

  

 Depth

4

1.0597

0.1929

0.012

0.007

 Residuals

32

4.4335

0.8071

  

Metagenomic data

 Host

9

0.1357

0.6726

0.001

0.008

 Residuals

22

0.0661

0.3274

  

 Diet

2

0.0497

0.2463

0.004

0.030

 Residuals

29

0.1521

0.7537

  

 Migration

2

0.0344

0.1706

0.024

0.001

 Residuals

29

0.1674

0.8295

  

 Depth

3

0.0620

0.3073

0.003

0.017

 Residuals

28

0.1398

0.6927

  
  1. Data were transformed into Bray–Curtis dissimilarities for analysis. P values for permutational MANOVA and permutation tests for homogeneity of multivariate dispersions are shown. Significant tests (p ≤ 0.05) are indicated in bold.
  2. df degrees of freedom, SS sum of squares, R2 coefficient of determination (proportional explained variation), p p value.
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