Fig. 1: GrowMiDE: Double emulsion platform for bacterial cultivation.
From: Double emulsions as a high-throughput enrichment and isolation platform for slower-growing microbes
A Schematic representation of the GrowMiDE platform. Four syringe pumps drive oil and aqueous solutions into a custom microfluidic device to encapsulate single microbes within 30 or 45 µm diameter double emulsions (DEs); DE generation is monitored in real-time by a high-speed camera attached to an Amscope stereoscope. B Schematic and representative brightfield image of DE droplets (scale bar = 30 µm). C Merged brightfield and fluorescent images of single E. coli-GFP cells loaded into DEs (left) and after growth in M9 + glucose for 24 h (right). D Brightfield microscopy images indicating growth of diverse anaerobes within DEs including the sulfate-reducer Desulfovibrio ferrophilus IS5 on 60 mM lactate and 30 mM NaSO4, the acetogen Thermoanaerobacter kivui on 50 mM glucose at 65 °C, lactic acid-producing fermenter Lactococcus lactis NZ9000 on 50 mM glucose, and mixed acid fermenter E. coli MG1655 on 50 mM glucose.
