Fig. 1: Microbiome sampling schematic, multivariate analyses, and relative abundances of dominant taxa.
From: Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome
Microbiome samples were collected from net-spinning caddisfly (Hydropsychidae) larvae and their filtration nets and retreats (“bio-structures”) and compared to those from adjacent rock-attached (control) biofilms (A). Microbiome taxonomic composition was compared by nonmetric multidimensional scaling (NMDS) analysis of unweighted UniFrac distances based on t1 (April 7, B) and t2 (June 2, C) 16 S rRNA gene ASV datasets. Final stress values for 3-d solutions are listed in (B, C). Mean percentage abundances of five dominant genera (1% or greater average 16 S rRNA gene amplicon relative abundance across t1 and t2) (D–M) with significant variation (p < 0.05; ANOVA with Tukey’s HSD post-hoc testing) in abundance between either nets or retreats versus rock biofilms at t1 (D–H) and t2 [42]. Genera are Rhodoferax (D, I), Flavobacterium (E, J), Luteolibacter (F, K), Pseudanabaena (G, L), and Methylotenera (H, M). Vertical axes are percentage abundance with identical scales. In (D–M), the bold line is the sample mean; the boxed region is the interquartile range (IQR); top and bottom whiskers indicate [Q3 + 1.5*IQR] and [Q1–1.5*IQR] respectively; and outliers are marked by open circles.
