Fig. 2: Comparison of SSU rRNA alignment rates of diverse viromes and EV preparations. | ISME Communications

Fig. 2: Comparison of SSU rRNA alignment rates of diverse viromes and EV preparations.

From: Extracellular vesicles are the main contributor to the non-viral protected extracellular sequence space

Fig. 2

Each dot represents the percentage of reads aligning to either 16S or 18S rRNA genes. The cyan line indicates the average alignment rate for publicly available metagenomes from various environments [19]. “Culture EV enrichment”: a cell-free preparation of EVs from Prochlorococcus cultures [33]. “Heligoland peDNA”: dataset generated in this study from a highly purified (filtration, DNase treatment, gradient purification) <0.2 µm Heligoland water fraction. “Environmental EV enrichment”: Density gradient-purified EVs isolated from seawater samples [33]. “GDOCB virome”: <0.2 µm fraction enriched for VLPs by flow cytometry [42]. “Tara Oceans virome”: <0.2 µm fraction purified by size filtrations and DNase treatment [17]. “Heligoland phage Isolates”: DNA extracted from virus isolates, purified by 0.2 µm size filtration [41].

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