Fig. 3: Temporal dynamics of CAZymes in RWS and TWS metaproteomes.

A Impact of the incubation time in the CAZyme dynamics of RWS and TWS metaproteomes, assessed by Multivariate Integrative Partial Least Square Discriminant Analysis (MINT-PLS-DA) based on CAZyme abundances (family level, CLR-transformed data). MINT-PLS-DA component 1 and 2 explained 30% and 16% of the total variance. Ellipses at 95% confidence. B Clustered Image Map (CIM) represented the most discriminant CAZyme families of the different sampling times in RWS and TWS. CIM was built using the main CAZyme families explaining the first two MINT-PLS-DA dimensions. Hierarchical clustering (Euclidean distance and Ward method) represents CAZymes in columns and samples (T1–T4) in rows. The boxes on the left highlights the clusters discriminating the sampling points (yellow, gray, purple and dark-red). The abundance of CAZy families is indicated by the white-to-red color gradient (increasing values).