Fig. 2: An overview of Ocean Genomes workflows.
From: Ocean Genomes: reference genome resources for marine vertebrates
Step 1—Sample acquisition, including identifying target species, engagement with relevant stakeholders to identify opportunities and strategy for sampling, voucher specimen and sample collection, expert identification, sample, specimen and metadata accessioning. Step 2—DNA and/or RNA extraction from tissue samples & quality assessment. Step 3—PacBio HiFi, Hi-C and transcriptome (Illumina RNA-Seq and PacBio Iso-Seq) library preparation & quality assessment. Step 4—Whole Genome Sequencing via short (Illumina) or long-read (PacBio HiFi) technologies & quality assessment. Step 5—Draft or reference quality genome assembly, quality assessment and manual curation. Step 6—Sequencing data and associated assemblies are openly accessible via custom and established public sequence repositories (Table 1).
