Fig. 3: Transcriptional analysis of top-performing β-lactams. | npj Antimicrobials and Resistance

Fig. 3: Transcriptional analysis of top-performing β-lactams.

From: A large screen identifies beta-lactam antibiotics which can be repurposed to target the syphilis agent

Fig. 3

a RNA was extracted and purified from T. pallidum cultures treated with 5 nM of the 23 β-lactams identified in Fig. 2c and analyzed by qRT-PCR using two targets: flaA (white) and tp47 (black). Purified genomic DNA and delta Ct values were used to calculate the number of copies of each target present in each treatment. Post amplification, melting curves were used to confirm the validity of the signal for each target. The diluents, water, and DMSO, were included as positive controls and a mono-culture of SfEp1 cells served as a negative control. All samples were assessed in duplicate and the mean (±s.d.) are shown. b Correlation analysis of tp47 and flaA qRT-PCR values attained in a. c The flaA and tp47 values were averaged for each treatment and grouped based on the 25% lowest samples, relative to the remainder. Statistical analysis, Mann–Whitney U test (****p < 0.0001).

Back to article page