Table 3 Bliss independence models of two-way and three-way combinations.

From: Synergy between Winter Flounder antimicrobial peptides

Two-way combinations

Three-way combinations

Condition

% Inhibition

Additive threshold

Bliss net inhibition

p value

Condition

% Inhibition

Additive threshold

Bliss net

p value

WF1/WF1a

4.26

10.02

−5.76

>0.9999

WF1/WF1a/WF1a1

61.16

26.53

34.62

0.0393

WF1/WF1a1

39.62

21.98

17.64

0.4673

WF1/WF1a/WF2

98.79

16.69

82.10

<0.0001

WF1/WF2

36.06

12.14

23.92

0.1006

WF1/WF1a/WF3

23.58

10.02

13.56

0.9892

WF1/WF3

−1.61

5.47

−7.07

0.9995

WF1/WF1a/WF3

2.63

11.18

−8.54

>0.9999

WF1/WF4

21.45

6.63

14.82

0.7274

WF1/WF1a/WF4

65.6

28.66

36.94

0.0212

WF1a/WF1a1

64.23

21.06

43.17

0.0002

WF1/WF1a1/WF2

73.56

21.98

51.58

0.0003

WF1a/WF2

71.21

11.23

59.98

<0.0001

WF1/WF1a1/WF3

35.09

23.14

11.95

0.9976

WF1a/WF3

4.46

4.55

−0.09

>0.9999

WF1/WF1a1/WF4

87.45

12.14

75.31

<0.0001

WF1a/WF4

−1.57

5.71

−7.28

0.9993

WF1/WF2/WF3

28.07

13.30

14.77

0.9736

WF1a1/WF2

37.72

23.19

14.53

0.7531

WF1/WF2/WF4

11.34

6.63

4.71

>0.9999

WF1a1/WF3

82.53

16.51

66.01

<0.0001

WF1a/WF1a1/WF2

99.24

27.74

71.50

<0.0001

WF1a1/WF4

15.96

17.67

−1.71

>0.9999

WF1a/WF1a1/WF3

92.80

21.06

71.73

<0.0001

WF2/WF3

97.77

6.68

91.10

<0.0001

WF1a/WF1a1/WF4

60.11

22.22

37.88

0.0164

WF2/WF4

12.86

7.83

5.03

>0.9999

WF1a/WF2/WF3

98.70

11.23

87.48

<0.0001

WF3/WF4

−1.16

1.16

−2.32

>0.9999

WF1a/WF2/WF4

78.73

12.39

66.34

<0.0001

     

WF1a1/WF2/WF3

99.16

23.19

75.97

<0.0001

     

WF1a1/WF2/WF4

36.94

24.35

12.59

0.9955

     

WF1a1/WF3/WF4

71.08

17.67

53.41

0.0002

     

WF2/WF3/WF4

99.47

7.84

−91.64

<0.0001

  1. The percentage inhibition of K. pneumoniae NCTC 13368 in MHB grown in the presence of two-way or three-way combinations of the six WF AMPs according to the method of Tekin et al.19. Subtraction of the additive threshold—inhibition expected from adding the contributions from non-interacting WF AMPs—from the inhibition achieved provides the Bliss net inhibition. Two-Way ANOVA with Šídák’s multiple comparisons test identifies combinations (shown in bold) where the % inhibition significantly surpasses the additive threshold and are synergistic, which for two-way (but not three-way) combinations is emergent. % inhibition and additive thresholds are the average of three independent replicates.
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