Fig. 1: A K. pneumoniae CFE platform from laboratory and clinical isolates. | npj Antimicrobials and Resistance

Fig. 1: A K. pneumoniae CFE platform from laboratory and clinical isolates.

From: A cell-free strategy for host-specific profiling of intracellular antibiotic sensitivity and resistance

Fig. 1: A K. pneumoniae CFE platform from laboratory and clinical isolates.The alternative text for this image may have been generated using AI.

a Overview of components and stages of the CFE reaction. b K. pneumoniae ATCC 13882 CFE activity of batch reactions (visual images) and real-time measurement of mScarlet-I (μM) concentration (calculated from a reference standard) over 14 h. Data is the mean and standard deviation of two biological repeats. c Workflow for CFE extract processing of clinical strains. d, e A heatmap representing the optimisation of mScarlet-I (μM) synthesis from ST258-T1b and f, g NJST258-1 clinical CFE systems with Mg-glutamate and K-glutamate. Two independent repeats (left and right panels) are shown from four biological repeats. Error bars represent the standard deviation of three measurements for each extract.

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