Fig. 1: Comparison of inhibitory effects of ribosome-targeting antibiotics on growth among the wild-type and the three ribosome rescue factor-deficient strains.
A Growth of the strains in the presence of various antibiotics. At the starting point, each strain was inoculated into 25 ml of LB medium at 37 °C in a 50 ml tube with a filter screw cap at a starting OD600 of 0.001. The LB medium contains each antibiotic at the IC50 concentration. After 5 h of growth in a shaking incubator, the OD600 of each culture was measured. It should be noted that the OD600 values of the wild-type strain after 5-h growth in the presence of each antibiotic at the IC50 concentration (particularly, tetracyclines and amphenicols) were not half of those in its absence, as the IC50 values were determined based on data after 8-h growth (Supplementary Fig. 2). Data are presented as the mean ± standard deviation of five independent experiments. Asterisks indicate significant differences compared to wild-type (Student’s t test, *p < 0.01; **p < 0.001). B Antibiotic concentration-dependent inhibition of growth of the wild-type and the three ribosome rescue factor-deficient strains. Each culture medium contained an antibiotic at the indicated concentration, and the OD600 of each medium was measured after 8 h of growth.
