Fig. 2: RNA sequencing data for M. abscessus treated with transition metal ions.
From: Transition metal homoeostasis is key to metabolism and drug tolerance of Mycobacterium abscessus
a Schematic workflow of RNA sequencing data extraction, quality control and data analysis used in this study. b PCA plot showing the distribution for three bacterial conditions, each with three biological replicates. c Volcano plots showing the distribution of genes for each ion-treatment group compared with the untreated control, filtered with cutoff fold change (log2FC > 2) and levels of significance (p < 0.05). d Gene set enrichment analysis (GSEA) for Co2+- and Ni2+-treated M. abscessus cultures compared with untreated bacteria. The relative size of each dot on the graph shows the number of differently regulated genes in each pathway, and the colour indicates the level of significance. The relative position on the × axis (gene ratio) denotes the proportion of differently expressed genes in a given pathway, in the range of 0–1.
