Fig. 1: Screening the COVID Box identified compounds active against intracellular Mtb. | npj Antimicrobials and Resistance

Fig. 1: Screening the COVID Box identified compounds active against intracellular Mtb.

From: The Parkinson’s drug benztropine possesses histamine receptor 1-dependent host-directed antimicrobial activity against Mycobacterium tuberculosis

Fig. 1

a Scatter plot showing the distribution of inhibitory effects of compounds (50 µM each) from the COVID Box library on intracellular Mtb in THP-1 macrophages 72 h post-infection. Green circles represent hits that inhibit Mtb growth by more than 70% with less than 25% cytotoxicity. b Seven hits with >70% intracellular Mtb growth inhibition (blue bars) and minimal cytotoxicity (red bars) were identified. Two are established antibiotics (doxycycline and tetracycline), while the other five were newly identified. c Chemical structures of the five newly identified hits. d Dose-dependent activity of BZT (IC50 = 15 µM) against intracellular Mtb. e CFU counts of Mtb after 72 h of infection in THP-1 macrophages. NT non-treated control. Data represent the mean ± SEM of at least two independent experiments. Statistical significance was determined using an unpaired T-test; **p < 0.005, *p < 0.05.

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