Fig. 1: Colistin persistence and dSTORM visualisation of bacterial envelope structures.
From: Lipopolysaccharide clustering in colistin persistent and resistant bacteria
A Biphasic time-kill curves of Ec1655 and PA14 treated with colistin. Ec1655 was exposed to 16 μg ml-1 and PA14 to 64 μg ml-1 colistin. Bacterial survival was assessed at 0 min, 1 h, 2 h, 4 h, 6 h, and 24 h, showing a characteristic biphasic killing profile with an initial rapid decline in colony counts, followed by persistence of a small surviving population. Data represent the mean ± SD of three biological and technical replicates (n = 3). Reconstructed dSTORM images and computational maps of bacterial cells stained with B FM4-64 to visualise membrane lipids and C rhodamine B-labelled polymyxin B (RB-PMB) to visualise LPS distribution. Images were reconstructed from 6000 raw frames using Huygens Localizer software. Representative images of untreated susceptible cells and colistin persisters are shown for both Ec1655 and PA14. The scale bars are 1 µm.
