Fig. 2: Skewed membrane distribution in susceptible and persistent E. coli and P. aeruginosa.
From: Lipopolysaccharide clustering in colistin persistent and resistant bacteria
A Illustration of fluorophore distribution preparation for skewness analysis. The bacterial membrane region was selected from dSTORM images. Scale bars are 2 µm. In the grey value plot, the abscissa represents the 20 × 20 nm2 area perimeter along the bacterial membrane, while the ordinate indicates the corresponding grey value intensity of fluorophores. Violin plots showing the skewness indices of B FM4-64-labelled membrane lipids and C RB-PMB-bound LPS along the cell envelope. Each plot includes data from 20 individual bacterial cells per condition. Plot lines indicate the first quartile, median, and third quartile. The horizontal dotted line denotes a skewness value of zero, representing a normal distribution. The significance test is an unpaired t test with Welch’s correction. NS (non-significant), P > 0.05; ***, P ≤ 0.001.
