Table 1 Strains used in this study including their genotype and their minimum inhibitory concentrations
Designation in main text E. coli strains | Genotype | Minimum inhibitory concentration | Origin of Strain, Reference | |||
|---|---|---|---|---|---|---|
AMP (µg ∙ mL−1) | CIP (µg ∙ mL−1) | COL (µg ∙ mL−1) | GEN (µg ∙ mL−1) | |||
MG1655 (wildtype, WT or ancestor) | Δ776 bp in crl, +8 bp bamD → / → raiA, +GC gltP → / ← yjcO | 4 | 0.01 | 0.5 | 0.5 | R. Mutzel lab (Blattner et al., 1997) |
S1 | Δ26,518 bp in lpxL | 4 | 0.02 | 0.5 | 0.5 | Nordholt et al.19 |
S2 | Δ6,314 bp in lpxL | 8 | 0.02 | 0.5 | 0.5 | |
S3 | rssB, lpxM | 8 | 0.02 | 0.5 | 0.5 | |
S4 | rssB, lpxM | 8 | 0.02 | 0.5 | 0.5 | |
S5 | opgC /opgG, lpxM | 4 | 0.02 | 0.5 | 0.5 | |
S6 | opgH, lpxM | 4 | 0.02 | 0.5 | 0.5 | |
WT-mCherry | attTn7::npII-mCherry | n.d. | n.d. | n.d. | n.d. | this study |
WT-YFP | attTn7::npII-YFP | n.d. | n.d. | n.d. | n.d. | this study |
S4-mCherry | attTn7::npII-mCherry | n.d. | n.d. | n.d. | n.d. | this study |
S4-YFP | attTn7::npII-YFP | n.d. | n.d. | n.d. | n.d. | this study |
WT∆ompF | Δ216bp in ompF | n.d. | 0.18 | n.d. | n.d. | this study |
S4∆ompF | Δ216bp in ompF | n.d. | 0.16 | n.d. | n.d. | this study |
BW25113 (wildtype) | ΔlacZ, ΔaraBAD, ΔrhaBAD, rph, rrnB, hsd, | n.d. | 0.02 | n.d. | n.d. | Keio collection (Baba et al., 200637); MIC determination this study |
JW1844 (ΔlpxM) | BW25113 lpxM::FRT-Kan-FRT | n.d. | 0.02 | n.d. | n.d. | Keio collection (Baba et al., 200637); MIC determination this study |
