Table 3 Description of plasmids, antibiotic resistance genes (ARGs) and transfer frequency (TF) of the Transconjugants produced in this study

From: Antimicrobial resistance, conjugative plasmids and pathogenicity in wastewater and freshwater Escherichia spp. in Stockholm, Sweden

Trans-conjugant

Plasmids in donor

ARGs in donor

TF

Plasmids transferred

ARGs transferred

Accession numbers

Amino-glycosides

β-lactams

Tetra-cycline

Sulfon-amides

Quino-lones

Trime-thoprim

18-CV601

IncFIB(AP001918), IncN, ColRNAI

blaCTX-M-15, blaTEM-1B, dfrA14, floR, mdf(A) qnrS1, tet)(A).

1.33 × 10−2 ± 0.00587

IncN

 

blaCTX-M-15

  

qnrS1

dfrA14

SAMN54758342

41-CV601

IncFIB(AP001918), IncFII, IncI1

aph(3”)-lb, aph(6)-ld, blaCTX-M-15, dfrA8, mdf(A), qnrS1, sul2

3.31 × 10−6 ± 0.00000124

IncI

 

blaCTX-M-15

  

qnrS1

 

SAMN54758343

42-CV601

IncFIB(AP001918), IncFII(pCoo), IncFII, IncI1, IncI2

blaCTX-M-1, mdf(A)

4.97 × 10−5 ± 0.0000497

IncI1

 

blaCTX-M-1

    

SAMN54758344

46-CV601

IncFIB(AP001918), IncFII, IncN

blaCTX-M-15, dfrA14, mdf(A), qnrS1

1.24 × 10−3 ± 0.0000713

IncN

 

blaCTX-M-15

  

qnrS1

dfrA14

SAMN54758345

47-CV601

IncFIB(AP001918), IncFIC(FII), IncI1, ColRNAI, Col8282

blaCTX-M-3, mdf(A)

1.12 × 10−3 ± 0.000589

IncI1, Col8282, ColRNAI

 

blaCTX-M-3

    

SAMN54758346

49-CV601

IncN

blaCTX-M-15, dfrA14, mdf(A), qnrS1

1.59 × 10−3 ± 0.000910

IncN

 

blaCTX-M-15

  

qnrS1

dfrA14

SAMN54758347

50-CV601

IncFII, IncP1, Col8282, Col156

blaCTX-M-15, mdf(A), qnrS1

1.77 × 10−5 ± 0.0000112

IncFII, IncP1, Col156

 

blaCTX-M-15

  

qnrS1

 

SAMN54758348

65-CV601

IncFII, Col440I, Col440II, ColRNAI, Col8282, Col(MG828)

aph(3”)-lb,aph(6)-ld, blaCTX-M-15, mdf(A), qnrS1, tet(B)

1.16 × 10−5 ± 0.00000581

IncFII

 

blaCTX-M-15

  

qnrS1

 

SAMN54758349

75-CV601

IncFIA, IncFIB(AP001918), IncFIC(FII), IncY, IncX1, IncX3

mdf(A), tet(A)

1.44 × 10−7 ± 0.0000000563

IncFIA, IncFIB(AP001918), IncFIC(FII)

  

tet(A)

   

SAMN54758350

76-CV601

IncFIA, IncFIB(AP001918), IncFIC(FII), IncY, IncX1, IncX3

mdf(A), tet(A)

1.44 × 10−7 ± 0.0000000563

IncFIA, IncFIB(AP001918), IncFIC(FII)

  

tet(A)

   

SAMN54758351

77-CV601

IncFIA, IncFIB(AP001918), IncFIC(FII), IncY, IncX1, IncX3

mdf(A), tet(A)

1.44 × 10−7 ± 0.0000000997

IncFIA, IncFIB(AP001918), IncFIC(FII)

  

tet(A)

   

SAMN54758352

79-CV601

IncFIA, IncFIB(AP001918), IncFIC(FII), IncY, IncX1, IncX3

mdf(A), tet(A)

1.76 × 10−7 ± 0.000000142

IncFIA, IncFIB(AP001918), IncFIC(FII)

  

tet(A)

   

SAMN54758353

80-CV601

IncFIA, IncFIB(AP001918), IncFIC(FII), IncY, IncX1, IncX3

mdf(A), tet(A)

2.47 × 10−7 ± 0.000000228

IncFIA, IncFIB(AP001918), IncFIC(FII)

  

tet(A)

   

SAMN54758354

81-CV601

IncFIA, IncFIB(AP001918), IncFIC(FII), IncY, IncX1, IncX3

mdf(A), tet(A)

2.12 × 10−7 ± 0.000000118

IncFIA, IncFIB(AP001918), IncFIC(FII)

  

tet(A)

   

SAMN54758355

82-CV601

IncFIA, IncFIB(AP001918), IncFIC(FII), IncY, IncX1, IncX3

mdf(A), tet(A)

1.76 × 10−7 ± 0.0000000815

IncFIA, IncFIB(AP001918), IncFIC(FII)

  

tet(A)

   

SAMN54758356

84-CV601

IncFIB(pB171), IncFII(pHN7A8), Col8282, Col440I

aph(3”)-lb, aph(6)-ld, blaTEM-1B, mdf(A), sul2, tet(B)

1.99 × 10−5 ± 0.0000154

IncFII(pHN7A8)

aph(3”)-lb, aph(6”)-ld

blaTEM-1B

tet(B)

sul2

  

SAMN54758357

  1. The columns show the transconjugant name (original donor code – CV601); plasmids and antibiotic resistance genes (ARGs) found in the original donors; transfer frequency (TF); plasmids and ARGs transferred to CV601 from WWTP (18, 41, 42, 46, 47, 49, 50, 65, 84) and Fresh/Brackish water (75, 76, 77, 79, 80, 81, 82) isolates. The TF displayed is the mean with standard deviation.
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