Table 2 SUP items commonly used for cell culture experiments in cancer research laboratories.

From: Towards greener and more sustainable pre-clinical oncology research

SUP

SUP type

Recyclable

Supplier (catalogue number)

Liquid handling

Stripettes part plastic wrap (5 ml, 10 ml, 25 ml)

PS / EVA1

Yes2

Fisher

(5 ml, 10420201; 10 ml, 10084450; 25 ml, 1060615)

Stripettes all plastic wrap (5 ml, 10 ml, 25 ml)

PS3

Yes2

Fisher (5 ml, 11829660; 10 ml,11839660; 25 ml, 11517752)

Pipette tips (P10, P200, P1000)

PP

Yes2

Starlab (P10, S1111-3700; P200, S1111-1706; P1000, S1111-2721)

Tissue culture vessels

Flasks (T25, T75)

PS

Yes2

Greiner (T25, 658175; T75, 660175)

Petri dishes (35 mm, 100 mm diameter)

PS

Yes2

Greiner (35 mm, 627160; 100 mm, 664160)

Multi-well plates (6, 12, 24, 48, 96 well)

PP

Yes2

Nunc (6-well Nunc; 1011983)

Corning (12-well ;10253041 48- well; 10732552)

Greiner (24-well, 662160; 96-well, 655180)

Containers for tissue culture solutions

Tissue culture medium

PET

Yes

Sigma (RPMI 1640, R8758-500ML)

Life Technologies (DMEM GlutaMAX, Gibco 11594446)

Phosphate-buffered saline

PET

Yes

Sigma (P4417)

Foetal bovine serum

PET

Yes

Biosera (FB-1092)

Trypsin

PET

Yes

Sigma (T3924-500ML)

Tubes

Eppendorf tubes (0.2, 0.5, 1.5 ml)

PP

Yes2

Fisher (0.2 ml, 1178110)

Axygen (0.5 ml, MCT-060-C; 1.5 ml MCT-175-C)

Universal (30 ml)

PP

Yes2

Sterilin (12521299)

Bijou (5 ml)

PS

Yes2

Sterilin (11369123)

Cryovial (1.8 ml)

PP

Yes2

Starlab (E3110-6122)

Miscellaneous

Autoclave bags

PP

Yes

Fisher (11309103)

Product outer packaging

Various

Mostly

Multiple

  1. PS Polystyrene, PP Polypropylene, PE Polyethylene, PET Polyethylene terephthalate, EVA Ethylene Vinyl Acetate.
  2. 1Outer wrapper is part PS, part EVA and requires separation into different recycling streams.
  3. 2Initially sterile but treated as hazardous waste after being in contact with cancer cells.
  4. 3Recycling costs are higher than for other SUPs [30].