Fig. 4: IL-6 and IL-10 are induced after anti-CD3/CD28 stimulation in PBMCs from patients with NSCLC.

a PBMCs supernatants from 19 NSCLC patients were collected. Cells were either untreated (UN) or treated with anti-CD3/CD28 antibodies. The a–e levels of IL-6, IL-10, IFN-gamma, and IL-2 in supernatants from PBMCs of patients with NSCLC were determined by multiplex analysis. The level of sSlamf1 was measured by ELISA. f–h Correlation analysis between IFN-γ and IL-10, IL-2 and IFN-γ, and between IL-2 and IL-10 levels in supernatants. It was found that, after activation of PBMCs from NSCLC patients, the immunosuppressive cytokine IL-10 correlated with levels of the effector cytokines IL-2 and IFN-gamma. These two latter cytokines correlated as well. Data are shown as mean values ± SEM using Student’s two-tailed t test. *p < 0.05; **p < 0.01; ***p < 0.001.