Fig. 6: Blocking PD1 function reduced Blimp-1 mRNA expression, while blocking Slamf1 function resulted in an induction of Blimp-1 mRNA levels and the immunosuppressive cytokine IL-10 in PBMC. | BJC Reports

Fig. 6: Blocking PD1 function reduced Blimp-1 mRNA expression, while blocking Slamf1 function resulted in an induction of Blimp-1 mRNA levels and the immunosuppressive cytokine IL-10 in PBMC.

From: The transcription factor Blimp-1 is suppressed by SLAMF1 and drives Treg cell-mediated immune evasion in non-small cell lung cancer

Fig. 6

a Experimental design in cell culture using PBMCs (48 h). Slamf1 function was blocked with anti-CD150 antibodies, while PD1 function was targeted with anti-PD1 antibodies (n = 4 patients). b Blimp1 mRNA expression was analyzed by qPCR and normalized by HPRT analysis. While anti-PD1 antibody treatment reduced Blimp-1 levels, anti-CD150 antibody treatment markedly induced Blimp-1 expression. c, d Release of soluble Slamf1(measured by ELISA) in different experimental conditions after 24 and 48 h culture. e Significant positive correlation between soluble Slamf1 protein and Blimp-1 mRNA expression. f Production of IL-2 after different stimulations was measured by multiplex analysis. g Levels of CD25/HPRT mRNA were measured in different experimental conditions. h, i IL-6 and IL-10 production in different experimental conditions were measured by multiplex analysis. j Activated TGF-beta was measured after activation in the supernatants by ELISA. Data are shown as mean values ± SEM using Student’ two-tailed t test. *p < 0.05; **p < 0.01; ***p < 0.001.

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