Fig. 1: Customized touchscreen chamber and DLC-VFA analysis.

A To increase the clarity of the video recording, the cover of the touchscreen chamber was replaced by a transparent wall booster (red dotted box). Additionally, the default house lights in the chambers were replaced with LED light strips, which were attached to the top drawer (red arrows). Additionally, the camera position was moved to the center of the chamber. B The top-down video was used for DLC and VFA analysis. In the illustrated video frame, DeepLabCut identified key anatomical points: the nose (marked with a red dot), the left ear (blue dot), and the right ear (green dot). Utilizing these points, the VFA package calculated the percentage of the stimulus area (the center hole area on the screen, marked with orange) that falls within each visual field area of the mouse. The green line is the center line of visual field. The area between blue lines is the binocular visual field. The areas between the blue line and yellow line are the monocular left or right visual fields. The screenshots show examples of frames when a mouse is oriented and not oriented to the screen.