Fig. 4: Enzymatic reusability for enCOH with LDG.
From: Molar-scale formate production via enzymatic hydration of industrial off-gases
a, Schematic of the experimental procedure. Enzymes with a His6-tag were immobilized on Ni-NTA resin for ten cycles of reuse, with no severe loss in conversion rate. The reaction was performed at room temperature (rt) using a rubber-sealed serum bottle (115 ml) with LDG-purged reaction buffer (20 mM EVox, 200 mM sodium phosphate buffer pH 6.5 with purged LDG gas for 1 h). The immobilized enzyme was subsequently recovered through a disposable open column, washed twice with 200 mM sodium phosphate buffer pH 6.5 and reused after each reaction. b, Repeated reuse of enzymes for enCOH with LDG. By using CODH and FDH, immobilized on Ni-NTA agarose beads, the reusability of the enzymes was tested. CO (circles) consumption, CO2 (triangles) and formate (reverse triangles) production, and yield (%, diamonds) were measured every 12 h (n = 1 replicate). Shaded bands denote the ten cycles.
