Fig. 1: LC-MS-based analysis of major ovarian hormone plasma levels. | npj Women's Health

Fig. 1: LC-MS-based analysis of major ovarian hormone plasma levels.

From: Mimicking women’s endocrine milieu in mice for women’s health-related studies

Fig. 1

a Illustration of the workflow of the optimized LC-MS method for simultaneous measurements of four ovarian hormones in plasma samples from humans and mice as small as 100 μl. Plasma samples are prepared using solid-phase extraction (SPE) for LC-MS analysis of P4 and T. The remaining samples are derivatized and analyzed by LC-MS for E2 and E1. Created in BioRender. Matvienko, D. (2024) BioRender.com/h27d558 with modifications. b Table showing lower limits of detection (LLOQ), % Relative standard deviation (RSD), % bias at LLOQ and calibration ranges for the different analytes, E2, E1, P4, and T.

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