Fig. 3: PGT145 binding leads to highly localized protection from deuterium exchange in the V2 loop but does not alter dynamics or structure throughout the rest of the trimers. | npj Viruses

Fig. 3: PGT145 binding leads to highly localized protection from deuterium exchange in the V2 loop but does not alter dynamics or structure throughout the rest of the trimers.

From: An HIV-1 broadly neutralizing antibody overcomes structural and dynamic variation through highly focused epitope targeting

Fig. 3

a Profile of local dynamics with and without PGT145 Fab bound to the well-ordered CNE55 trimer shown in “butterfly plot” where each point corresponds to a peptide (mid-point of the peptide segment indicated) at a given time point across the primary sequence. The PGT145 footprint effect is clearly observable in the difference plot shown below. A difference heatmap of changes in deuterium exchange is shown on the right mapped onto pdb 5ACO. Equivalent data for PGT145 Fab binding to BJOX2000 trimer shown in (b). Deuterium uptake plots for peptides reflecting PGT145-induced effects shown for CNE55 trimer (c) and for BJOX2000 trimer (d).

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