Fig. 4: Single-particle cryo-EM reconstructions of two divergent Env native-like trimers with PGT145 Fab reveals engagement of glycans from multiple gp120 protomers is required for PGT145 binding. | npj Viruses

Fig. 4: Single-particle cryo-EM reconstructions of two divergent Env native-like trimers with PGT145 Fab reveals engagement of glycans from multiple gp120 protomers is required for PGT145 binding.

From: An HIV-1 broadly neutralizing antibody overcomes structural and dynamic variation through highly focused epitope targeting

Fig. 4

a Side and top view of cryo-EM map with fitted atomic model of BJOX2000 Env+PGT145 Fab. gp120 and gp41 chains are colored magenta and gray. b Side and top view of cryo-EM map with fitted atomic model of CNE55 + PGT145. gp120 and gp41 chains are colored purple and sky blue. In all panels, heavy and light chain of the PGT145 Fab are colored orange and green respectively. Interactions of PGT145 with N160 glycans are shown in close-up panels. HCDR: Complementarity determining region of Fab heavy chain, LCDR: Complementarity determining region of Fab light chain, FR: Fab framework region.

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