Fig. 5: Comparison of N156 Glycan density in the Cryo-EM at low and high threshold. | npj Viruses

Fig. 5: Comparison of N156 Glycan density in the Cryo-EM at low and high threshold.

From: An HIV-1 broadly neutralizing antibody overcomes structural and dynamic variation through highly focused epitope targeting

Fig. 5

a Cryo-EM map of BJOX2000 + PGT145 fitted with atomic model is shown at different thresholds. In case of N156 glycan, interactions are seen only between chain A of Env and HCDR2 of Fab. Clear density connecting Fab and N156 glycan at chain A in the case of BJOX2000 + PGT145 is seen at lower thresholds. In higher threshold also, concerned residues are close enough to interact. b Cryo-EM map of CNE55 + PGT145 fitted with an atomic model is shown at different thresholds. In the case of the N156 glycan, interactions are seen only between chain A of Env and HCDR2 of Fab. In the case of CNE55 + PGT145, there is no clear connecting density between N156 glycan and Fab at high threshold, but the concerned residues are close enough for possible interactions. In all panels, density that corresponds to N156 glycan is shown as dotted circle, though glycan chain was not built into density considering the resolution limitation.

Back to article page