Fig. 1: Determination of the matrix metalloproteinase (MMPs) activities.
From: Novel mechanism of the COVID-19 associated coagulopathy (CAC) and vascular thromboembolism
The zymographic analysis of the sera samples collected from mice that were either administered with or without SARS-CoV-2 spike protein (SP) intra-renally into the cortex. The samples were loaded on to the gelatin (upper panel) and casein (lower panel) gels. Lanes are labeled as: hACE2 (no SP), and hACE2+SP (with SP), samples were loaded in duplicates with the 2× concentration and 1× concentrations respectively. Collagenase was used as a standard control. Relative levels of the gelatinase (MMP-2 and MMP-9) and the caseinase; MMP-13 and ADAMTS13) activities are shown. The bar graphs are representations of the scan units from the gels, represented as the Mean ± SD, n = 4, SP, * p < 0.05.
