Fig. 2: Estimation of the NGAL and MMP-9 levels.
From: Novel mechanism of the COVID-19 associated coagulopathy (CAC) and vascular thromboembolism
The renal cortex MMP-9 and NGAL activities were determined by gelatin (left upper gel panel) using the gel zymography. Lanes are labeled as: Control (C) i.e., the hACE2 mice (no SP), 4wks+SP. An intra-renal (into the cortex) injection was used for administering the SARS-CoV-2 spike protein (SP), and the samples were collected at 2, 4 wks, and at 4wks+SP + AG (treatment and untreated with aminoguanidine; AG an iNOS inhibitor). The sera samples from mice (hACE2 1×, 2× and hACE2+SP 1×, 2×) were used for MMP-13 quantitation using the zymography (bar graph). The bar graphs are representations of the scan units from the gel’s panels, Mean ± SD, n = 4, * and ** p < 0.05. PUMP putative/truncated metalloproteinase.
