Fig. 4: Phylogenetic tree of the “LSCV” M Segment.

A maximum-likelihood tree was generated using a General Time Reversible model based on nucleotides of the M segment (glycoproteins Gn/Gc coding region) of multiple reported sequences of orthohantaviruses. There are 1,000 bootstraps supported. MSB:Mamm:332771 (red) indicates reference genome generated. Samples from New Mexico formed a monophyletic clade composed of two subclades, delineating further geographic structure and containing samples from Site 1 (blue), Site 2 (green), and Site 3 (gold). Additional orthohantavirus reference sequences were obtained through NCBI. The scale bar represents the number of nucleotide substitutions per site. US United States, MX Mexico, TX Texas, SLP San Luis Potosí, NL Nuevo León, EM State of Mexico, AZ Arizona, NM New Mexico.