Fig. 1: EBOV replication kinetics in Leydig cells and Sertoli cells.

Quantitation by RT-PCR of VP40 gene copies in cell pellet and supernatant of Leydig cells (A) and Sertoli cells (B) treated with infectious EBOV. Primer sequences for strand-specific RNA reverse transcription and quantitation of the VP40 gene are listed in Table 1. The cutoff for minimum threshold of detection was at 36 cycles. Each data point represents the average of three biological replicates, for each of which two technical replicates were performed during quantitation. For each cell type, significant changes in viral load between neighboring time points were determined using the Student’s t test. Asterisks indicate a significant change in viral load, where *, **, ***, and **** indicates that P ≤ 0.05, P ≤ 0.01, P ≤ 0.001, and P ≤ 0.0001, respectively.