Table 1 Sequences for primers used during reverse transcription and quantitation of RNA collected from infectious EBOV-treated and EBOV trVLP-treated Leydig cells and Sertoli cells

From: Characterizing changes in transcriptome and kinome responses in testicular cells during infection by Ebola virus

Primer target

Primer sequence (5’ to 3’)

PCR type

Tm (°C)

Strand-specific vRNA

GGCCGTCATGGTGGCGAATGGTGAATGTCATATCGGGCCC

Reverse transcription

62.5

Primer tag

GGCCGTCATGGTGGCGAAT

Real-time qPCR

65.1

VP40 (reverse)

GATGGCGGCCGTAGTTGAG

 

62.4

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