Fig. 2: Distribution of ACE2 and TMPRSSII in the upper and lower respiratory tract. | npj Viruses

Fig. 2: Distribution of ACE2 and TMPRSSII in the upper and lower respiratory tract.

From: Jamaican fruit bat (Artibeus jamaicensis) insusceptibility to mucosal inoculation with SARS-CoV-2 Delta variant is not caused by receptor compatibility

Fig. 2: Distribution of ACE2 and TMPRSSII in the upper and lower respiratory tract.

Anti-ACE2 immunohistochemistry (A–D, I–L) and anti-TMPRSS2 immunohistochemistry (E–H, M–P) in k18 humanized mice, Syrian golden hamster, mink, and Jamaican fruit bats. Nasal turbinates: A k18 mouse, ACE2 immunoreactivity along the ciliated border of the respiratory epithelium lining the nasal turbinate (>), within rare sustentacular cells of the olfactory epithelium (*) and frequently within olfactory gland cells. There is a non-specific “blush” staining of the submucosa and nasal cavity exudate. E k18 mouse, TMPRSS2 immunoreactivity is present in the sustentacular cell layer of the olfactory epithelium (*) and olfactory gland cells but absent in the ciliated respiratory epithelium (>). B Hamster, ACE2 minimal apical immunoreactivity of the ciliated respiratory epithelium (>) and a near absence of olfactory epithelial immunoreactivity (*). F Hamster, TMPRSS2 immunoreactivity is absent in the ciliated respiratory epithelium (>) but abundant in the sustentacular cell layer of the olfactory epithelium (*) and the glandular epithelium within the submucosa. C Mink, ACE2 immunoreactivity of the ciliated respiratory epithelium (>) and absence of olfactory epithelial immunoreactivity (*). G Mink, TMPRSS2 immunoreactivity is absent in the ciliated respiratory epithelium (>) but abundant in the sustentacular cell layer of the olfactory epithelium (*) and occasionally the olfactory neuroepithelium. D Jamaican fruit (Aj) bat, ACE2 immunoreactivity along the ciliated border of the respiratory epithelium lining the nasal turbinate (>) but absent within the olfactory epithelium (*). H Bat, TMPRSS2 immunoreactivity is present in the sustentacular cell layer of the olfactory epithelium (*) and glandular epithelium but absent in the ciliated respiratory epithelium (>). There is also immunoreactivity of the nasal cavity contents, which are believed to be the apical surface of adjacent cells and a result of the function of the cut. Lung: I. k18 mouse, ACE2 immunoreactivity spans the apical border of the ciliated and non-ciliated terminal bronchiolar epithelial cells (>). M k18 mouse, TMPRSS2 immunoreactivity is both cytoplasmic and nuclear within columnar cells lining the bronchioles (>). J Hamster, ACE2 immunoreactivity is absent from the bronchiolar epithelium (>). N Hamster, TMPRSS2 immunoreactivity is abundant within the bronchiolar epithelium (>). K Mink, ACE2 immunoreactivity of the apical aspect of the bronchiolar ciliated respiratory epithelium (>). O Mink, TMPRSS2 immunoreactivity is absent in the bronchiolar ciliated respiratory epithelium (>). L Bat, ACE2 immunoreactivity of the bronchiolar ciliated respiratory epithelium (>). P Bat, TMPRSS2 immunoreactivity of columnar epithelial cells (>) juxtaposed with mucus-containing goblet cells.

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