Fig. 1: Single-cell measurements of VEEV replicon RNA amplification in Vero cells. | npj Viruses

Fig. 1: Single-cell measurements of VEEV replicon RNA amplification in Vero cells.

From: Venezuelan equine encephalitis virus non-structural protein 3 dictates superinfection exclusion in mammalian cells

Fig. 1: Single-cell measurements of VEEV replicon RNA amplification in Vero cells.The alternative text for this image may have been generated using AI.

a Graphical illustration of the VEEV replicon (VEEVrep) RNA, and virus genomes (VEEV and SINV-eGFP) used in this study. b Schematic representation of experimental approach. VRP-mCherry (10 VRPs/cell) and/or VRP-eGFP (10 VRPs/cell) were transduced in Vero cells. Twenty-four hours post transduction, cells were fixated and Hoechst stained. Fluorescence microscopy images were analyzed using a customized CellProfiler pipeline to quantify mCherry-, eGFP-, and dual-positive cells of the total number of Hoechst-stained cells. Image generated with Adobe Illustrator using Office Word icons. c Percentage of mCherry- and eGFP-positive cells after single and co-transductions of VRPs. Bars represent the mean and standard deviation of two independent experiments with each of eight biological replicates, ****P < 0.0001(detailed information in Supplementary Table 1).

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