Fig. 3: Viremia and viral load in the brain of C57BL/6 WT mice inoculated with different doses of WNV-578/10 and WNV-NL20 via the intradermal route.
From: In vitro and in vivo characterization of a novel West Nile virus lineage 2 strain
Statistical analyses were done using a 2-way ANOVA (alpha = 0.05) (n = 5 mice per group). A Viral load in the blood as determined by qPCR. Shown as max. Ct (45) minus the measured Ct value. Blood was sampled every 2 days post-infection up until 12 dpi, or when the humane end-point was reached. Significant differences over day 2–8 pi are indicated with asterisks as follows: WNV-NL20 high dose vs WNV-578/10 low dose 8 dpi *p = 0.0101, WNV-NL20 high dose vs WNV-578/10 high dose 8 dpi *p = 0.0186. Error bars represent the SEM. B Infectious virus titers in different regions of the brain of mice that were euthanized when the humane end-point was reached, as measured by virus titration. Shown as Log10TCID50 per gram of tissue. LOD = limit of detection. Significant differences between WNV-578/10 high dose vs. WNV-NL20 low dose (p value* = 0.0381) and WNV-578/10 high dose vs. WNV-NL20 high dose (p value* = 0.0449) were found for titers in the frontal lobe, as indicated by the asterisks.
