Fig. 1: Differential lung response induced by RSV in neonates compared to adults.

Female 6–7 weeks old (adult) or mixed 7 days old (neonates) BALB/c mice were infected with RSV. Infection schedule made with BioRender (A). Percentage all CD8 Trm (B) and RSV specific (C). Airway cells were transferred from neonatal RSV-infected mice into naïve adult mice prior to the RSV challenge of the recipient mouse, weight at d7 (D). In a separate study, neonatal and adult mice were infected with RSV, on day 7, lungs were collected from the infected animals and RNAseq was carried out from RNA extracted. PCA of genes in different groups (E), pathways related to each PC (F) and loading genes driving PC2 and PC3 are shown in (G). Volcano plots of DEG from Adult (H) and Neonatal (I) mice. Bars in B and C represent mean ± SEM of n = 5 mice. *p < 0.05.