Fig. 4: Boosting primary RSV infection with chemokines enhanced tissue resident TRM production and confer protection. | npj Viruses

Fig. 4: Boosting primary RSV infection with chemokines enhanced tissue resident TRM production and confer protection.

From: Modulating cytokine microenvironment during T cell activation induces protective RSV-specific lung resident memory T cells in early life in mice

Fig. 4

Seven-day-old BALB/c mice were infected intranasally with RSV, and 20 µg of CCL5 or CXCL10 in 20 μl were given on days 7, 8 and 9 after infection. Mice were culled on day 21, and flow cytometry was performed on the lungs that were harvested from the animals. Lung cell count (A), CD8 (B), Trm epithelial CD8 T cells (C). In a separate study, neonatal mice were infected with RSV and subsequently received chemokines intranasally on d7, 8 and 9 of infection before re-challenge on day 21. Mice were followed for 4 days after infection, and weight loss (D), lung cell count (E), CD8 TRM% (F), Viral Load (G) and antibody (H) were recorded. The same set-up was repeated except CCL5 or CXCL10 was given only on day 7 post primary infection. Mice were followed for 4 days after infection, and weight loss (I), lung cell count (J), CD8 TRM% (K), Viral Load (L) and antibody (M) were recorded. *p < 0.05, **p < 0.01, ***p < 0.001; statistical analysis by one-way ANOVA except for panels D and I where 2 way ANOVA. N = 5 mice per study.

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