Fig. 3: The E293K mutation in the E protein of USUV Af-3 does not lead to attenuation in mice.
From: Dose and strain dependent lethality of Usutu virus in an Ifnar−/− mouse model
a Ifnar−/− mice were inoculated subcutaneously with 100 pfu of rAf-3-WT (n = 8), rAf-3-E293K (n = 8) or with DMEM (n = 3), weighed daily and tail bled on alternating days. b Survival rates for each of the experimental groups. Statistical analysis was performed using the log-rank (Mantel-Cox) test. c Daily weight loss measured as a percentage of initial weight for each of the experimental groups showing mean ± SEM. Statistical analysis was performed using a one-way ANOVA for each time point.
