Table 1 Summary of earlier studies assessing USUV lethality in immunocompromised mouse models
From: Dose and strain dependent lethality of Usutu virus in an Ifnar−/− mouse model
Study | Model | Age/Sex | Inoculation/Dose | Lineage + isolate (Host species) | Survival Results |
|---|---|---|---|---|---|
Segura Guerrero et al.10 | IFN α/β/γ receptor−/− (129) | 8–14 wk Male | IP 1 × 106 pfu | Eu-* strain V18/BH65/11-02-03(TM) | 100% Lethal Day 2–3 |
IP 1 × 105 pfu | 100% Lethal Day 3 | ||||
IP 1 × 104 pfu | 70% Lethal Day 4 | ||||
IP 1 × 103 pfu | 100% Lethal Day 4–5 | ||||
IP 1 × 102 pfu | 75% Lethal Day 4 | ||||
IP 1 × 101 pfu | 70% Lethal by Day 9 | ||||
Bates et al.11 | CD-1 (anti-Ifnar1) | 3 wk Male | Inj.* 1 × 105 pfu | Af-3 UG09615/UG-2012 (Cx) | 40% Lethal Day 10 |
Af-3 TMNetherlands-2016/NL-2016 (TM) | Non-lethal. | ||||
Salgado et al.12 | CD-1 (anti-Ifnar1) | 8 wk Male + Female | SC Footpad 1 × 103 pfu | Af-2 HU10279-09/SP-2009 (Cx) | No morbidity or weight loss in either strain |
Af-3 UG09615/UG-2012 (Cx) | |||||
Martín-Acebes et al.13 | Ifnar−/− (129) | 6 wk Male + Female | IP 1 × 104 pfu | Af-2 Biotec (Vero-derived SAAR1776) | 90% Lethal Day 5–10 |
Clé et al.14 | Ifnar−/− (B6) | 8–12 wk Male | IP 1 × 104 TCID50 | Af-2 Rhône2705/FR-2015 (TM) | 100% Lethal Day 6 |
Constant et al.15 | Ifnar−/− (*) | Adult | Subdermal 1 × 103 TCID50 | Eu-2 TE20421/IT-2017 (TM) | 100% Lethal Day 3–5 (Show neuroinvasion). |
Kuchinsky et al.16 | Ifnar−/− (B6) | 8 wk Male | SC footpad 1 × 103 pfu | Af-2 SAAR-1776/SA-1959 (Cx) | 100% Lethal Day 5–6 |
Af-2 HU10279-09/SP-2009 (Cx) | 100% Lethal Day 5–6 | ||||
Af-3 DakPM173701/SE-2003 (Cx) | 100% Lethal Day 4–5 | ||||
Af-3 UG09615/UG-2012 (Cx) | 100% Lethal Day 5–6 | ||||
Af-3 TMNetherlands 2016/NL-2016 (TM) | 10% Lethal Day 7 | ||||
Study | Model | Age/Sex | Inoculation/Dose | Lineage(s) | Results |
Salgado et al.12 | Ifnar−/− (B6) | 10–18 wk Male + Female | SC Footpad 1 × 103 pfu | Af-2 HU10279-09/SP-2009 (Cx) | 100% Lethal Day 7 |
Af-3 UG09615/UG-2012 (Cx) | 100% Lethal Day 5 (SP-2009 shows lower viremia titres) | ||||
Bates et al.11 | Ifnar−/− (B6) | 3 wk Male | Inj.* Footpad 2 × 105 pfu | Af-3 UG09615/UG-2012 (Cx) | 100% Lethal Day 3 |
Af-3 TMNetherlands-2016/NL-2016 (TM) | 100% Lethal Day 6 | ||||
This study | Ifnar−/− (B6) | Adult Male + Female | SC Hind limb 1 × 102 pfu | Eu-2 Bologna-2009/IT-2009 (Hu) | 100% Lethal Day 4 |
Eu-3 AS201700077/NL-2017 (TM) | 100% Lethal Day 5 | ||||
Af-3 TMNetherlands/NL-2016 (TM) | 100% Lethal Day 6 | ||||
2 × 101 pfu | Af-3 TMNetherlands/NL-2016 (TM) | 90% Lethal Day 8 |
