Table 2 Load of virus RNA, immunohistochemistry (IHC) for nucleoprotein, and severity of microscopic lesions in the central nervous system from 6 young (Y) turkeys that were inoculated intramuscularly with aquatic bird bornavirus 1 (virus-inoculated, VI) at 1 day of age, and which presented with neurological signs

From: Pathogenesis of aquatic bird bornavirus 1 in turkeys of different age

Turkey number

Cohort

dpib

Clinical signsc

Virus RNAd

IHC

Inflammatione

Serologyf

Brain

Spinal cord

Gonad

Brain

Spinal Cord

Brain

Spinal cord

T442a

Y, VI

63

ATX, REC

3.88

6.60

1.2

-

-

1.0 Bs,Cr(g)

2.0Lu

0.317

T443a

Y, VI

64

ATX, REC

4.88

5.94

1.2

-

-

0.5 Bs

2.0Lu

0.167

T444a

Y, VI

65

ATX

5.41

5.82

1.2

-

-

0.0

2.0Lu

0.184

T445a

Y, VI

65

ATX, REC

2.50

6.00

1.2

+Bs

+Lu

0.0

3.0Lu

0.258

T452

Y, VI

84

ATX

7.17

7.72

1.65

+ Bs,Cr

-

2.0 Bs,Cr,Cl,Ol

2.0Lu

0.638

T454

Y, VI

84

ATX

6.92

6.91

1.2

+ Bs,Cr,Cl,Ol

+Cr,Th,Lu

2.3 Bs,Cr,Cl,Ol

2.0Cr,Th,Lu

0.546

  1. aIndicates that the bird was euthanized outside of sampling point (emergency bird).
  2. bDays post-inoculation (dpi). All birds developed neurological signs after 8 weeks post-inoculation (wpi), and were included in the 12 wpi tally for the other group analyses.
  3. cClinical sign: ataxia/inability to stand (ATX), sternal recumbency (REC).
  4. dReported as log10 copy number for 150 ng of total RNA; if negative, reported at the limit of detection (1.2).
  5. eInflammation, reported as organ scores for brain and spinal cord (range: 0-6).
  6. fReported as normalized optical density (OD) values, with a cut-off OD = 0.261.
  7. gIndicates which sections of the brain, or segments of the spinal cord were reactive for IHC, or from which scores were calculated: brainstem, Bs; cerebrum, Cr; cerebellum, Cl; optic lobe, Ol; cervical, Cv; thoracic, Th; lumbar, Lu.
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