Fig. 1: Humoral responses against NL63 spike in healthy subjects.
From: Potent neutralising monoclonal antibodies targeting the spike of NL63 coronavirus
a The table depicts plasma IgG endpoint titres against NL63 full length spike and the S1B domain (RBD), neutralising titres against the Amsterdam1 live virus isolate in HAT24 cells, and frequencies of NL63 spike-specific memory B cells (MBC). Subjects are ranked in order of NL63 spike-specific MBC frequencies. The two subjects that mAbs were isolated from are highlighted in yellow. The ELISA and neutralisation data were from two technical replicates. b Representative flow cytometry plots of NL63 and SARS-CoV-2 spike-specific memory B cells from two subjects stained with recombinant spike proteins fluorescently labelled with PE or APC. c Frequencies of NL63 and SARS-CoV-2 spike-specific memory B cells as a proportion of CD19+ IgD- IgG+ B cells in PBMCs (N = 14). The line and error bars depict median and interquartile range respectively. Statistical analyses between matched pairs were performed with a Wilcoxon signed-rank test (***P < 0.001). Results in this figure are representative data from single experiments.
