Fig. 4: Amino acid conservation within NL63 spike.
From: Potent neutralising monoclonal antibodies targeting the spike of NL63 coronavirus
A Amino acid conservation across NL63 strains (N = 207 sequences spanning 1983—2023 from Genbank) is illustrated on a monomer of NL63 spike (PDB: 8FR7). Shading indicates areas of low ( < 80%; deep red), partial ( > 80%; salmon), near complete ( > 0.98%; light pink) and complete conservation (100%; grey). B Amino acid conservation depicted on NL63 domain S1B (RBD) in complex with the ACE2 receptor (PDB: 3KBH). C Amino acid sequence conservation of the residues around the escape mutations (in black boxes) for the S2 neutralising mAb (NLH02) and three RBD neutralising mAbs (NLH04, NLH07 and NLH18). Residues are shaded according to how well conserved they are with orange/red indicating lower conservation.
