Fig. 4: A nonsynonymous SNP in the P gene expedites the growth of BoDV-2.
A, B BoDV-2 minireplicon assays using the indicated BoDV-2 P variants and BoDV-2 N and (A) LRG or (B) LRGTR as helper plasmids. Data are presented as the means ± SEM of three biologically independent experiments. An unpaired t-test was performed for statistical analysis. ns, not significant; **p < 0.01. C–F Growth kinetics assay of rBoDV-2 variants. Vero cells infected with the indicated rBoDV-2 were cocultured with uninfected Vero cells at a ratio of 1 to 19 and passaged every 3 or 4 days. DPC, days post coculture. C, D The percentage of infected cells was determined by IFA using an antibody against BoDV N. E, F The copy number of viral RNA was measured via RT-qPCR using total RNA extracted from each cells. The data are presented as the means ± SEM of three biologically independent experiments. Two-way ANOVA and Tukey’s or Sidak’s multiple comparisons test were performed for statistical analysis. **p < 0.01; ns, not significant.
