Table 1 Primers used in the 5’ and 3’ RACE analysis for BoDV-2
Target | RT primer | PCR primer | |
|---|---|---|---|
Forward | Reverse | ||
5’ end of genome | 5’-GCCTTATTTAAGCCCGTTTACAGG | 5’-CGACTGGAGCACGAGGACACTGA | 5’-GATGGACTTGGTCATGATTCGAGG |
3’ end of genome | 5’-CCAAAACGCCATTTCCACCTTCTCTTC | 5’-CCAAAACGCCATTTCCACCTTCTCTTC | 5’-GAGGATATCTCTAACTCGGTGAGC |
5’ end of antigenome | 5’-CAGCTCCAGCCTTAATCTTGG | 5’-CGACTGGAGCACGAGGACACTGA | 5’-GAGGATATCTCTAACTCGGTGAGC |
3’ end of antigenome | 5’-CCAAAACGCCATTTCCACCTTCTCTTC | 5’-CCAAAACGCCATTTCCACCTTCTCTTC | 5’-GATGGACTTGGTCATGATTCGAGG |
