Fig. 3: Principal component analysis of virus titer, protein abundances, and mRNA levels in mice shows tissue-related grouping.

Mice were inoculated with A/Bovine/Ohio/B24osu-342/2024 (BoV342, green) or A/Vietnam/1203/2004 (VN1203, blue) at 10² (circle), 10³ (square), or 10⁴ TCID₅₀ (triangle), via aerosols. Principal component analyses are shown for mice at 3 days post inoculation (a) and at endpoint (b) with K-means clusters differentiated by light gray polygons. The optimal number of clusters (two for day 3 and three for endpoint) were identified by the majority vote of 26 indices for determining the number of clusters⁵².