Fig. 4: In vitro characterization of LGTVDNA+L–2000 from passage 6 and LGTVLab in Vero and A549 cells.
A Percent cytotoxicity determined by lactate dehydrogenase (LDH) cytotoxicity assay at 96 hpi. The experiments were conducted in octuplicate (Vero) or sextuplicate (A549), and absorbance was measured in technical triplicates. B LGTV growth kinetics represented as RNA copies per mL of cell culture media. The experiments were run in triplicates. C LGTV growth kinetics represented as infectious virus titers at indicated time points. The experiments were run in duplicates. D LGTV infectivity represented as RNA copies to FFU ratios at indicated time points. Specific infectivities were calculated using the same samples and time points, run in duplicates. The error bars represent SEM, and significant differences are indicated (*p < 0.05, **p < 0.01).
