Fig. 4: Overexpression of JEV E protein. | npj Viruses

Fig. 4: Overexpression of JEV E protein.

From: Transcriptomic response of mosquitoes to Japanese encephalitis virus and identification of its potential entry factors

Fig. 4

A Western blot analysis of E. coli overexpressing JEV E protein at 0 h and 4 h after induction with IPTG. Bacterial lysates were run in duplicate, and the blot was then cut in half, where the molecular weight (MW) marker was included. Subsequently, the blots were probed using the two available monoclonal antibodies against flavivirus E proteins (4G2 and M2-8E7). B SDS-PAGE analysis of the overexpressed, purified E protein using the Ni-NTA resin beads.

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