Fig. 6: Tissue pathology and inflammatory cytokine in the SI of SARS-CoV-2 VOC-infected hamsters.
From: Gut infection and dysbiosis are hallmarks of severe SARS-CoV-2 variants
Hamsters (n = 3 to 4) were infected with 106 PFU/100 µL/animal of Hong Kong, Delta or Omicron VOCs. Animals were sacrificed at 4 dpi and SIs were collected and processed for histology. a Representative H & E-stained sections of duodenum of normal and different infected groups at 40x magnification showing the crypt depth and the villi (Yellow arrow). b Crypt depth and c villi length was quantified using ImageJ/Fiji. d Villi/crypt ratio was also measured. Data from 8–10 villi-crypt units per animal were measured. RNA was extracted from the SI and quantified the expression levels of IFN-ϒ e, IL-6 f, and TNF-α g by RT-qPCR. Statistics were performed by one-way ANOVA. *p < 0.05, **p < 0.01, ns - not significant. Error bars indicate Mean ± SEM.
