Fig. 1: Schematic representation of the ORFs and predicted sequence motifs of TiLV segments 1–10.
From: Characterisation of the tilapia lake virus proteome and identification of an 11th protein, S9-F3
UTRs are symbolised by horizontal black lines, and open boxes represent ORFs, approximately to scale. The primary coding regions are annotated (top bars) with predicted functional motifs (SDD, RdRp catalytic motifs (fingers, palm and thumb); NLS, nuclear localisation signal; SP, signal peptide; N-gly, N-linked glycosylation; TMD, transmembrane domain; NES, nuclear export signal). Structural domains of S1-S4, corresponding to the influenza polymerase subunits and NP respectively, and S8 were taken from PDB 8PSN, 9HBR and 8IXW, respectively. Other ORFs (minimum length 25 codons) starting with a methionine codon from the three frames (Fr) are also indicated. The box with dashed lines in S3 represents an ORF lacking AUG codons in the region corresponding to the X-ORF in IAV. AUG codons represented by vertical lines are coloured according to the predicted strength of their Kozak translation initiation context (green, strong; amber, intermediate; red, weak). The predicted major polypeptides are shown in frame 1 (Fr1) with black arrows indicating canonical AUGs predicted to be used for translation initiation of the primary TiLV protein.
