Fig. 5: Dependence of syncytia formation on cellular cholesterol levels. | npj Viruses

Fig. 5: Dependence of syncytia formation on cellular cholesterol levels.

From: STAT1 signaling controls cholesterol metabolism in epithelial cells and RSV-induced syncytia formation

Fig. 5: Dependence of syncytia formation on cellular cholesterol levels.The alternative text for this image may have been generated using AI.

A Fluorescence images from IncuCyte live-cell imaging of rRSV-A-0594-eGFP-infected wild-type (left) and STAT1−/− (right) HEp-2 cells (MOI 0.05), which were left untreated (left column), treated with 50 μM Gemfibrozil (GFZ, middle column), or with 10 μM 25-hydroxycholesterol (25-HC, right column) at 12, 24, 36, and 48 hpi. Images representative of three independent (two technical replicates) experiments. Scale bar indicated next to images. B Quantification of syncytia size by IncuCyte live-cell imaging for green (fluorescent) area per image relative to green object count per image of rRSV-A-0594-eGFP-infected wild-type (solid lines) and STAT1−/− (dashed lines) HEp-2 cells (MOI 0.05), either untreated (red), treated with 50 μM GFZ (blue), or 10 μM 25-HC (green). Images of cells were taken every hour for up to 48 hpi. Mean ± SEM of eight independent experiments (two technical replicates) is shown. Statistical analysis was conducted on the overall distribution of syncytia size during the course of infection using the Friedman test with Dunn’s multiple-comparison test.

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