Fig. 6: Surface expression of RSV PostF protein.

Confocal microscopy images of RSV-A-0594-infected wild-type (A) and STAT1−/− (B) HEp-2 cells (MOI 0.05) at 48 hpi. Infected cells were left untreated (top), treated with 50 μM Gemfibrozil (GFZ, middle), or 10 μM 25-hydroxycholesterol (25-HC, bottom). Cells were stained for RSV postfusion F protein on the cell surface. Intracellular actin was visualized by ActinRed staining and nuclei by NucBlue staining. Images representative of three independent experiments (two technical replicates). Scale bar indicated next to images.